relb c1e4  (Cell Signaling Technology Inc)

Journal: International journal of molecular sciences

Article Title: NF-κB Signaling Modulates miR-452-5p and miR-335-5p Expression to Functionally Decrease Epithelial Ovarian Cancer Progression in Tumor-Initiating Cells.

doi: 10.3390/ijms24097826

Figure Lengend Snippet: Figure 1. Volcano plot of differentially expressed miRNAs plotted with respect to the log2 Fold change (log2FC) and −log10 False discovery rate (−log10FDR) in OV90 cells with: (A) RELA-knockdown cells grown adherently, (B) RELB-knockdown cells grown adherently, (C) RELA-knockdown cells grown in spheroid conditions, (D) RELB-knockdown cells grown in spheroid conditions. The candidate miRNAs for this study, hsa-miR-452-5p and hsa-miR-335-5p, are identified as red and green circles, respectively. (E) Venn diagram of differentially expressed miRNAs in each condition.

Article Snippet: RelB antibody (C1E4) was from Cell Signaling Technologies (Danvers, MA, USA).

Techniques: Knockdown

Journal: International journal of molecular sciences

Article Title: NF-κB Signaling Modulates miR-452-5p and miR-335-5p Expression to Functionally Decrease Epithelial Ovarian Cancer Progression in Tumor-Initiating Cells.

doi: 10.3390/ijms24097826

Figure Lengend Snippet: Figure 3. Sphere-formation capability of OV90 and OVCAR8 with either RELA or RELB silenced. Spheres with an area greater than 1000 square microns were quantified. (A) OV90 spheres were transfected with

Article Snippet: RelB antibody (C1E4) was from Cell Signaling Technologies (Danvers, MA, USA).

Techniques: Transfection

Journal: International journal of molecular sciences

Article Title: NF-κB Signaling Modulates miR-452-5p and miR-335-5p Expression to Functionally Decrease Epithelial Ovarian Cancer Progression in Tumor-Initiating Cells.

doi: 10.3390/ijms24097826

Figure Lengend Snippet: Figure 4. Measured cell viability of EOC cell lines containing silenced RELA or RELB with modu- lated miR-452-5p expression. (A) Viability of OV90 spheres silenced for either RELA or RELB and transfected with either 90 pmol of miR-452-5p inhibitor or 0.1 pmol miR-452-5p mimic. (B) Viability of OVCAR8 spheres that were silenced for either RELA or RELB and transfected with either 90 pmol of miR-452-5p inhibitor or 0.1 pmol miR-452-5p mimic. Graphs represent mean with standard error of mean (SEM). Analysis was performed by using a two-way ANOVA and Dunnett’s multiple com- parisons test. ** p < 0.01, **** p < 0.0001, n.s.—non-significant as compared to the negative control (NC) within each cell line (shNeg, shRelA, shRelB).

Article Snippet: RelB antibody (C1E4) was from Cell Signaling Technologies (Danvers, MA, USA).

Techniques: Expressing, Transfection, Negative Control

Journal: International journal of molecular sciences

Article Title: NF-κB Signaling Modulates miR-452-5p and miR-335-5p Expression to Functionally Decrease Epithelial Ovarian Cancer Progression in Tumor-Initiating Cells.

doi: 10.3390/ijms24097826

Figure Lengend Snippet: Figure 5. ALDH activity in OV90 EOC cells with silenced RELA or RELB after miRNA modulation. (A) Quantified percentages of ALDH+ cells in RELA- or RELB-silenced OV90 spheres after adding 90 pmol of either negative control (NC) or hsa-miR-452-5p inhibitor (452 Ib). (B) Quantified percent- ages of ALDH+ OV90 cells with RELB silenced and transfected with 0.1 pmol miR-452-5p inhibitor (452 Ib). Graphs represent data from three independent experiments (n = 3) and are plotted to indicate mean with SD. Analysis was performed by using an unpaired t-test. ** p < 0.01, n.s.—non-significant as compared to negative control.

Article Snippet: RelB antibody (C1E4) was from Cell Signaling Technologies (Danvers, MA, USA).

Techniques: Activity Assay, Negative Control, Transfection